Lab Test 1 Study Guide

1.  Microscope parts and function

2.  Leukocytes and roles in immune response

3.  Bacterial Shapes - Know the terms for the common bacterial shapes.

4.  Smear Preparation - Air dry and heat fix, why?

5.  Staining      Acidic Stains - What is stained using the acidic stain?  Why?      Basic Stains - What is stained using the basic stain?  Why?      Mordant - What is(are) the purpose(s) of the mordant?      Decolorizer - What is the purpose of the decolorizer?

6.  Simple Stain      What can one learn about the cell by performing a simple stain?      What is a negative stain?

7.  Differential Stain      What can one learn about the cell by performing a differential stain?      Describe the Gram Stain procedure, including the reagents used,                   expected and erroneous reactions, and cell wall components.      Describe the Acid-Fast stain procedure including the reagents used,               expected and erroneous reactions, and cell wall components.

8.  Special Stains      Flagella Stain - What is function of the flagellum?      Monotrichous, Lophotrichous, Amphitrichous, Peritrichous      Capsule Stain      What combination of stains are used?  What is stained, what is not?      Why is the presence of a capsule significant?      Endospore Stain      Describe the Endospore stain procedure including the reagents used,         expected reactions, and cell wall components.  Why is the presence of      endospores significant?

9.  Colony Morphology and terms